HPLC Analysis of Garlic Clove Homogenates (continued)

Component	Source of Variation	Sum of Squares	Degrees of Freedom	Mean Squares	F	P-value
1-propenyl allyl/allyl 1-propenyl	Between Groups	232.02	2	116.01	61.4	6.00E-08
	Within Groups	28.35	15	1.89
	Total	260.37	17
diallyl	Between Groups	138.8	2	69.4	1.75	0.207
	Within Groups	593.7	15	39.6
	Total	732.5	17
methyl 1-propenyl/1-propenyl methyl	Betwwen Groups	12.35	2	6.17	11.35	9.96E-04
	Within Groups	8.16	15	0.54
	Total	20.51	17
2-propenyl methane/methyl 2-propene	Between Groups	139.6	2	69.8	3.27	0.066
	Within Groups	320.1	15	21.34
	Total	459.7	17
dimethyl	Between Groups	12.07	2	6.03	1.76	0.205
	Within Groups	51.4	15	3.43
	Total	63.47	17
total thiosulphinate	Between Groups	1.22E+11	2	6.08E+10	2.22	0.143
	Within Groups	4.10E+11	15	2.73E+10
	Total	5.32E+11	17

Table 3. Analysis of variance

Applying a t-test for paired groups it is found that the difference in levels of 1-propenyl allyl (+ allyl 1-propenyl) thiosulphinate is significant between isozyme groups 1 and 3 and between isozyme groups 1 and 4.

The t-test also shows that the difference in levels of 1-propenyl methane (+methyl 1-propenyl) thiosulphinate is significant between the same two paired groups.

Since the formation of thiosulphinates in garlic homogenates is the result of the rearrangement of sulphenic acids derived from the enzymatic breakdown of sulphoxides, the differences found between isozyme groups may be the function of the quantities of individual methyl, allyl and 1-propenyl groups available. Table 4 shows the breakdown of the individual alkyl groups and further statistical analysis (ANOVA) shows that it is the levels of the 1-propenyl group that account for the above group differences (Table 5).

Clone	Group	Total Allyl (%)	Total 1-Propenyl (%)	Total Methyl (%)	Total Area (equiv.)	Cloves (No)	Flowers (No)	Bulbils (No)	Scape Length (mm)	Bulb Weight (g)	Bulbil Weight (g)
Yugoslavian	1	74.82	6.52	18.67	815595	9	30	25	107	41	8.3
Israeli	1	74.47	7.5	18.04	648415	12	22	25	97	60	12
French Red	1	74.62	6.01	19.36	742744	6	16	4	91	32	5
Yerina	1	72.37	7.12	20.51	740736	9	28	13	109	43	7.7
German Red	1	69.34	7.99	22.69	728148	10	17	9	104	59	8.8
Dominics	1	81.41	6.56	12.04	719903	10	13	7	99	66	8.2
Roja	1	80.88	5.84	13.55	789610	10	35	28	94	43	6.6
Carpathian	1	79.84	4.6	15.58	604524	7	40	16	99	33	7.1
Music	3	69.72	0.92	29.35	974971	7	300	243	159	45	8.4
Georgia Fire	3	79.33	2.06	18.62	883232	6	290	208	153	38	6.2
Leningrad	3	76.02	1.94	22.04	1115679	7	273	302	152	55	10.7
Red Rezan	3	76.9	3.05	20.07	758803	7	154	105	88	20	3.2
Freudenberger	4	80.25	2.36	17.4	1073418	12	0	0	0	41	n/a
Morado	4	85.69	2.1	12.21	1030958	13	0	0	0	60	n/a
Red Janice	4	81.1	2.86	16.04	527125	8	0	0	0	32	n/a
California Late	4	77.1	1.77	21.15	857919	18	0	0	0	43	n/a
Cristo	4	78.81	2.08	20.77	960630	24	0	0	0	59	n/a
Inchelium	4	73.11	1.66	24.05	514311	14	0	0	0	66	n/a

Table 4. Summary of thiosulphinate content and morphological traits of garlic clones